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Methods The inhibitory effect of BHB was monitored as a result of insulin incubation in the presence of glucose or fructose using AGE-dependent fluorescence, Tyr fluorescence as well as anilinonaphthalenesulfonate (ANS) andthioflavin T (ThT) binding, and circular dichroism (CD) investigations. To study lipid peroxidation induced by insulin glycation, thiobarbituric acid (TBA) assay and thiobarbituric acid reactive substance (TBARS) monitoring were used. Fileboss V3 Cracked. The effect of insulin–AGE on microglial viability was investigated by 3-(4, 5 dimethylthiazol-2-yl)—2, 5-diphenyltetrazoliumbromide (MTT) cell assay and Annexin V/propidium iodide (PI) staining. Introduction Type 1 Diabetes is generally characterized by raised level of blood sugar (hyperglycemia) due to imperfection in insulin secretion, type 2 diabetes is characterized by insulin resistance that results in both of hyperglycemia and hyperinsulinemia and finally type 3 diabetes is characterized with neurodegeneration linked with insulin resistance. Although, increasing evidences in the literature pointing toward strong correlation between insulin resistance and Alzheimer’s disease (AD) [, ] but, that correlation has not been yet formally recognized.